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KMID : 0358320060470060656
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Korean Journal of Urology
2006 Volume.47 No. 6 p.656 ~ p.660
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The Anti-inflammatory Effect of Cobalt-Protoporphyrin for Rats with Epididymitis Induced by Escherichia coli Infection
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Seo Ill-Young
Jeong Chan-Sang
Rim Joung-Sik
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Abstract
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Purpose: Heme oxygenase-1(HO-1), an inducible heat shock protein, catalyzes the heme to iron, biliverdin and carbon monoxide. It also has an inhibitory effect on necrosis and inflammation. Cobalt(III)-protoporphyrin IX(CoPP) is known to be a HO-1 inducer. Our intension was to find whether CoPP has an anti-inflammatory effect through the induction of HO-1 in rats with epididymitis.
Materials and Methods: Thirty two Sprague-Dawley male rats(age: 8-12 weeks, weight: 200-250gm) were selected for the experiments. Anesthesia was performed with an intraperitoneal injection of ketamine hydrochloride(140mg/kg). Four rats were taken and used as a control group. Epididymitis was induced in 28 rats by an injection of E. coli(1x105/ml) to the epididymis. In the first step, groups of 4 rats were sacrificed serially after 4, 12, 48, and 72 hours for Hematoxylin & Eosin(H&E) staining and Western blot for inducible nitric oxide synthase(iNOS) and cyclooxygenase(COX)-2. In the second step, groups of 4 rats were injected with either dimethyl sulphoxide(DMSO) 7μl, DMSO 7μl with 50mg/ml CoPP or DMSO 7μl with 100mg/ml CoPP. They were then sacrificed 72 hours later for H&E staining and Western blot for iNOS and COX-2.
Results: In the first step, increased inflammation was evident from the H&E staining over time. From the Western blots, iNOS expression was detected after 48 hours and COX-2 was after 12 hours. In the second step, decreased inflammation was evident from the H&E staining, and the expressions of iNOS and COX-2 were suppressed in the CoPP treated group.
Conclusions: CoPP can reduce the inflammation of epididymis in rats, and the mechanism may be related with HO-1. (Korean J Urol 2006;47: 656-660)
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KEYWORD
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Cobalt protoporphyrin, Epididymitis, Heme oxygenase
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